Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 25 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area.
- Biochemical buffer
- Effective pH range: 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- Appearance: Solid
- Color: White to off-white
- Target: Biochemical assay reagents
- Pathway: Others
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661161.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 500 Grams
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent used as a pH buffer in research and laboratory applications. This 500-gram volume is ideal for the preparation of buffer solutions with stable pH levels, particularly for biochemical assays and protein analysis. BES is used widely in enzyme studies, especially where precise pH control is required. It is valued for its ability to maintain a consistent pH in solutions that mimic physiological environments. The reagent’s high buffering capacity ensures reliable performance in a variety of applications, including cell culture and chromatography processes, where pH balance is crucial for accurate results.
![Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660867.jpg-250.jpg)
Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram
TES, Sodium Salt, is a high-purity buffering agent available in a 1-kilogram package. This tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid sodium salt is widely used in biochemical and molecular biology research for maintaining stable pH conditions in solutions. TES buffers are particularly effective in biological and clinical applications where precise pH control is essential. The high purity of this reagent makes it suitable for sensitive experiments, including enzyme assays, cell culture, and PCR reactions. TES buffers are used to stabilize proteins, DNA, and other biomolecules, ensuring consistent and reliable results in a variety of laboratory protocols.
FUJIFILM Biosciences RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Selleck Chemical LLC HEPES
HEPES is a nonvolatile zwitterionic chemical buffering agent which is broadly applied in cell culture HEPES is effective at pH 6 8 to 8 2 HEPES is also a potent inducer of lysosome biogenesis
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 24 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer is designed for plant tissue culture and is available in a 5-liter size. This powder, containing 24 grams, makes 5 liters of solution, providing essential nutrients and pH stability with the inclusion of MES buffer. The buffer ensures the pH remains constant, which is vital for maintaining optimal growth conditions for plant cells. This medium is widely used in laboratories for plant cell culture, genetic studies, and regeneration research. MES buffer is particularly important in experiments that require precise pH control, making this medium an essential tool for researchers working with plant tissue cultures.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659833.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Free Acid is a commonly used biochemical reagent available in a 500g size for large-scale buffer preparation. This acid form of HEPES is highly effective in maintaining pH in the range of 6.8 to 8.2, making it ideal for biological applications where pH stability is crucial. It is commonly used in cell culture, protein analysis, and enzyme assays, ensuring that the pH remains constant over extended periods. HEPES is non-toxic to most cells, making it an excellent choice for maintaining physiological conditions during experiments. This bulk package is perfect for high-volume laboratories and applications that require frequent buffer preparation.
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 100G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 100G
Hampton Research StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 250G
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 250G
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659771.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 1 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a highly effective buffer used in a wide range of biochemical and molecular biology applications. This 1kg USP-grade bottle provides enough reagent for preparing larger quantities of pH-stable solutions. PIPES is particularly useful in situations where other commonly used buffers, such as phosphate, cannot maintain their pH under high salt conditions or at extreme temperatures. This buffer is essential for many laboratory protocols, including protein purification, enzyme assays, and cell culture studies. The large volume is perfect for high-throughput research and provides significant cost savings for laboratories that require large amounts of buffer solution.
