Life Science Buffers
Sigma Aldrich Fine Chemicals Biosciences Dulbecco’s Phosphate Buffered Saline, 1000 mL
Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process, where the presence of calcium and magnesium can inhibit trypsin activity. For this application, use DPBS Modified, which is formulated without calcium and magnesium salts.
United States Biological Corporation DULBECCO MEM W/O GLU PWD 10L
NC2682569 DULBECCO MEM W/O GLU PWD 10L
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Hampton Research HR2-729/1.0 M HEPES pH 7.5 - 100 ml
HR2-729/1.0 M HEPES pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Dojindo Molecular Technologies Inc MES (100G), Good's buffer, CAS: 145224-94-8, pka=6.15, Active pH 5.5-7, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Dojindo Molecular Technologies Inc MOPS (25G), Good's buffer, CAS: 1132-61-2, pka=7.2, Active pH 6.5-7.9, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
RIGAKU REAGENTS MES/ NaOH, 1M, pH6.0, 250 ml, CAS # 4432-31-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Genscript Corporation MES SDS Running Buffer Powder
MES SDS Running Buffer is formulated for running ExpressPlusᵀᴹ and SurePAGEᵀᴹ, Bis-Tris gels. This buffer is ideal for separating small- and medium- sized proteins. For separation of large- to medium- sized proteins, Tris-MOPS SDS buffer (M00138) is recommended.
Reconstitute with 1000 ml deionized water to make 1X running buffer per pack of powder. Do not use acid or base to adjust the pH.
eMolecules TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | 1179360-20-3 | MFCD23702017 | 1g
AstaTech | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | 1g | 412394943 | I11907 | 95.000 | 1179360-20-3 | MFCD23702017 | 266.770 | C11H23ClN2O3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Hampton Research Tris Buffer
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) Please note items from this supplier are non-returnable.